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In the present study the regeneration and establishment of whole plants free from pathogenic contaminants from kava (Piper methysticum Forster) using meristems tissue culture has been established. Four treatments for initiating kava in tissue culture were evaluated for their efficiency in eliminating contaminants and optimizing recovery and growth. One of the treatments, using shoot-tip meristems resulted in 84% decontamination with 72% recovery and another, using nodal bud meristems, resulted in 80% decontamination rate with only 20% recovery. Shoot tips were found to be the ideal explants for kava tissue culture. The minimum time taken for rooting was one month on Murashige and Skoog medium supplemented with 100 mgL-1 myo-inositol, 0.04 mgL-1 BAP, 0.02 mgL-1 NAA, 0.05 mgL-1 GA, 1.0 mgL-1 thiamine and 20 gL-1 sucrose.

Regeneration and establishment of whole plants from kava (Piper methysticum Forster) meristems in tissue culture