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This study was conducted to investigate the efficacy of FSH stimulation on the number of bovine oocytes retrieved by non-ultrasound-guided transvaginal ovum pickup and their developmental competence. In Experiment 1, to study the effect of FSH treatment on meiotic maturation, oocyte donors were divided into two groups (n = 5/group). Group I received no FSH treatment and aspiration was performed twice weekly, whereas Group II received 200 mg FSH (Folltropin-V) treatment twice daily for 3 days prior to aspiration, which was performed once every two weeks. Both groups were investigated for 8 weeks, after which crossover treatment continued for another 8 weeks. The retrieved oocytes were cultured for 24 h in vitro, and the chromosomal stages were evaluated by staining with 1% aceto-orcein. Experiment 2 was designed to determine the effect of FSH treatment on developmental competence of bovine oocytes. Oocytes were obtained from FSH-treated and untreated control cows (n = 3/group), and they were matured, fertilized, and cultured in vitro. The results showed that Group II females had a higher number of retrieved oocytes per cow per session than Group I females (7.05 ± 3.88 vs. 2.06 ± 0.99, respectively; P &lt; 0.05). The FSH stimulation led to an increase in the proportion of matured oocytes (69.6% vs. 63.3%) and also increased the percentage of cleavage stage embryos (49.4% vs. 32.2%) compared with untreated control cows (P &lt; 0.05). However, there was no difference between the two groups in percentage of morula and blastocyst formation (11.1%, 8.6% and 10.2%, 6.8%, respectively). In conclusion, the combination of non-ultrasound-guided transvaginal ovum pickup with FSH stimulation increased the number of retrieved oocytes per cow per session and tended to increase in vitro embryo production.The authors gratefully acknowledge the support by the Chiang Mai Artificial Insemination Center, Thailand.

reproduction, fertility, and development/reproduction, fertility and development

254 EFFECT OF FSH TREATMENT ON NUMBER OF BOVINE OOCYTES RETRIEVED BY NON-ULTRASOUND GUIDED TRANSVAGINAL OVUM PICK-UP AND THEIR DEVELOPMENTAL COMPETENCE