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The sequencing of ribosomal RNA and DNA (rRNA/rDNA) from environmental samples heralded a new age in microbiology. The advent of next-generation sequencing supercharged these methods, which now give high-resolution data sets, enabling real insights intomicrobial diversity and function in complex systems. Here, three local applications of 16S rDNA pyrosequencing are described, which highlight the usefulness of this approach for addressing practical questions in diverse areas of microbiology. Limitations of the sequence-based approachwill also be discussed.

Wastewater, wheat and table wipes: adventures in culture-independent microbiology