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International audienceA new approach based on measurements of nitrate and boron isotopic composition associated with microbiological indicators for the determination of nitrate origin in karstic groundwater (SW, France) is presented. Nitrate and boron isotopic data indicate an animal source of nitrate (delta N-15-NO3- > 5 parts per thousand, delta O-18-NO3- < 10 parts per thousand and delta B-11 similar to 25 parts per thousand). Microorganism detection (bacteriophages) confirmed contamination from animal sources and proved fast water transfer (2-3 days) from surface to groundwater

environmental chemistry

Combination of nitrate (N, O) and boron isotopic ratios with microbiological indicators for the determination of nitrate sources in karstic groundwater