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Although sodium fluoroacetate ("1080") is a poison useful for the control of rabbits it has two serious defects: one is its chemical stability due to the virtual indestructibility of the C-F linkage; and the other is that it is very poisonous for domestic stock. Consequently baits treated with "1080" must be covered or removed after a poisoning or, where that is not practicable, stock must be kept out of the poisoned paddocks until the baits are innocuous. Apart from the results of an unpublished study by Mr. D. Gooding of the Department of Agriculture in Western Australia on the effect of artificial rainfall on the "1080" content of poisoned oats. there is no information about the rate of decrease of "1080" in baits exposed to average weathering conditions. The following tests mere made to determine the point. The baits used were unhusked oats and fresh carrots. Sodium fluoroacetate was added to chopped carrot a t the rate of 0 . 3 mg/g wet weight and to oats a t the rate of 0 . 3 mg/g dry weight. (These proportions are comparable to those used in ordinary "1080"-poisoning practice.) The baits lay exposed to the weather, on the freshly mown surface of an open paddock a t "Gungahlin" near Canberra, from November 18. 1958 to January 20, 1959. The "1080" was assayed by subcutaneous injections of extracts of the baits into immature mice of 15 g weight. The number of deaths brought about by doses of 0 . 4 ml and 0 .2 ml of the extract was then compared with the number brought about by injection of standard doses of 0 . 2 and 0 . 1 mg of sodium fluoroacetate. Eight mice were injected a t each dose level and the potency was calculated according to the method of Marks and Pak (1935)$ for insulin assay. Adult mice are insensitive to small amounts of "1080n, but those of 15 g weight can be made sensitive if they are fasted 17 hours beforehand and kept a t 4°C for the duration of the test. By this method 0.05 mg of "1080" can be detected. Control mice fasted and injected with water were unaffected by cold a t 4OC. The extracts were prepared by macerating. in a Waring blender, 12 g of ovendried carrot or 50 g of oven-dried oats with 100 ml of distilled water. The bait was re-extracted with a further 50 ml of distilled water: each blending was squeezed

The effect of weathering on the toxicity of baits treated with Sodium Fluoroacetate