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The incorporation of fixed carbon from carbon dioxide into mouse embryos was greatest in eight-cell embryos which developed into blastocysts during a 24-hr culture period and was four to five times greater than the incorporation into two-cell embryos cultured for a similar period. The net accumulation of labelled products in the culture medium was greatest during the culture of the morula stage to the blastocyst over 24 hr.

Fixation of Carbon Dioxide by Pre-Implantation Mouse Embryos in Vitro and the Activities of Enzymes Involved in the Process