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Chloride influx measured with 36Cl over a 10 min loading period followed by a 5 min wash in inactive solution is used as an estimate of influx across the plasmalemma in barley roots. Chloride influx measured over a 60 min loading period followed by a 30 min wash is used as an estimate of the quasi-steady influx from the external solution to the vacuole

australian journal of biological sciences

The Use of Short-Term and Quasi-Steady Influx in Estimating Plasmalemma and Tonoplast Influx in Barley Root Cells at Various External and Internal Chloride Concentrations