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When phenyl ,B-D-thioglucopyranoside is added to a washed mycelial suspension of S. atra, strain CMI 32542, under conditions previously shown to result in the "inductive" synthesis of ,B-glucosidase, a series of other glycosidases and an esterase is concomitantly formed. The same enzymes are not formed in each experiment, but a series El, E2,  can be set up such that synthesis of En is not observed unless En-l is also synthesized. Induction by "homologous" inducers (e.g. phenyl ,B-D-thioxylopyranoside for ,B-xylosidase, phenyl &lt;X-D-thioglucopyranoside for &lt;x-glucosidase, etc.) is not observed. A certain slight increase in the activity of enzymes of this group is observed on prolonged (up to 24 hr) shaking of mycelial suspensions in the absence of any inducer. This increase is much less than that occurring in the presence of phenyl ,B-D-thioglucopyranoside, and the two phenomena can normally be easily distinguished.

Linked Induction of Glycosidases in Stachybotrys Atra