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The addition of casein and lecithin to a phosphate-saline diluent in which ram and bull spermatozoa were cooled to 5C increased their subsequent viability at 37C and the effects were additive. Casein and lecithin also increased the metabolism of fresh ram semen. After storage at 5C, oxygen uptake and oxidation of lactate by ram and bull spermatozoa were lower than before storage. The decrease in the amount of fructose oxidized to carbon dioxide was greater.

Effect of Casein, Lecithin, Glycerol, and Storage At 5°C on Diluted Ram and Bull Semen