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Methods are described for obtaining preparations of isolated spinach chloro-plasts which are predominantly either "whole", i.e. retain the outer limiting membrane, or "naked", i.e. with the outer membrane removed. The whole chloro-plasts are shown to retain more of their soluble components as evidenced by higher endogenous capacities for CO.-fixation, photophosphorylation, and light-dependent reduction of triphosphopyridine nucleotide. The isolation is also described of whole chloroplasts whose peripheral jackets have retained the property of mobility seen in living cells.

Biochemical and Structural Correlations in Isolated Spinach Chloroplasts Under Isotonic and Hypotonic Conditions