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Measurements of ion exchange were made between isolated cell walls of Ohara australis and an external solution. Comparison between intact cells and cell walls showed that nearly all the easily exchangeable cations are located in the cell wall. The wall is  hown to consist of "water free space" (W.F.S.) and "Donnan free space" (D.F.S.); the concentration of in diffusible anions in the D.F.S. is about O 6 equivjl. This finding is contrary to past suggestions that the D.F.S. is in the cytoplasm of plant cells.

Ionic Relations of Cells of Ohara Australis I. Ion Exchange in the Cell Wall