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The glycolysis of ram semen was measured at 37, 21, and 5°C. Measurements of QI0 over this range gave values between about 2 and 3 for changes in lactic acid and total reducing substances. Cold shock greatly reduced glycolysis and dehydrogenase activity which were only partially maintained by egg yolk and lecithin.

The Effects of Incubation Temperature and Cold Shock on the Metabolism of Ram Spermatozoa