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We demonstrate a non-stereoscopic, video-based particle tracking system withoptical tweezers to study fluid flow in 3D in the vicinity of glass nanopores.In particular, we used the Quadrant Interpolation algorithm to extend ourvideo-based particle tracking to displacements out of the trapping plane of thetweezers. This permitted the study of flow from nanopores oriented at an angleto the trapping plane, enabling the mounting of nanopores on a micromanipulatorwith which it was then possible to automate the mapping procedure. Mapping ofvoltage driven flow in 3D volumes outside nanopores revealed polarity dependentflow fields. This is in agreement with the model of voltage driven flow inconical nanopores depending on the interaction of distinct flows within thenanopore and along the outer walls.

3D Flow Field Measurements Outside Nanopores