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This paper presents for the first time an innovative instrument called aninverted scanning microwave microscope (iSMM), which is capable of noninvasiveand label-free imaging and characterization of intracellular structures of alive cell on the nanometer scale. In particular, the iSMM is sensitive to notonly surface structures, but also ectromagnetic properties up to one micrometerbelow the surface. Conveniently, the iSMM can be constructed throughstraightforward conversion of any scanning probe microscope, such as the atomicforce microscope or the scanning tunneling microscope, with a simple metalprobe to outperform traditional SMM in terms of ruggedness, and width,sensitivity and dynamic range. By contrast, the application of the traditionalSMM to date has been limited to mainly surface physics and semiconductortechnology, because the traditional SMM requires a fragile and expensive probeand is incompatible with saline solution or live biological cells.

Inverted Scanning Microwave Microscope for In Vitro Imaging and Characterization of Biological Cells