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Phalaenopsis Orchid Homeobox1 (POH1) gene is a KNOTTED1 homologous gene from Indonesian wild orchid Phalaenopsis amabilis. As a member of homeobox gene family in orchid, POH1 may function in the initiation and development of shoot in orchid. To understand the roles of POH1 gene, we analyzed the expression and function of POH1 gene during in vitro culture of P. amabilis orchids. The method was conducted by growing seeds of P. amabilis orchids on New Phalaenopsis (NP) medium to grow protocorms, and then in turned it grew into plantlets. Phenotypic characters such as a number of shoots, number and size of leaves were analysed in (4, 8, 16 and 24) wk after sowing (WAS) plantlets. Molecular analysis including the expression of POH1 gene was analysed by Reverse Transcriptase (RT)-PCR using POH1F1R1 primers on various ages of (4, 8, 16 and 24) WAS plantlets following by protein profile analysis using 10 % SDS-PAGE. The results showed that during in vitro condition, embryos were developed into a single and multip...

Dynamic expression of POH1 gene in shoot development during in vitro culture of Phalaenopsis orchid