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We have developed a modular system for high-resolution microscopy at highhydrostatic pressure. The system consists of a pressurised cell of volume ~100microlitres, a temperature controlled holder, a ram and a piston. We have madeeach of these components in several versions which can be interchanged to allowa wide range of applications. Here, we report two pressure cells with pressureranges 0.1-700MPa and 0.1-100MPa, which can be combined with hollow or solidrams and pistons. Our system is designed to work with fluorescent samples(using a confocal or epifluorescence microscope), but also allows fortransmitted light microscopy via the hollow ram and piston. The system allowsprecise control of pressure and temperature [-20-70C], as well as rapidpressure quenching. We demonstrate its performance and versatility with twoapplications: time-resolved imaging of colloidal phase transitions caused bypressure changes between 0.1MPa and 101MPa, and imaging the growth ofEscherichia coli bacteria at 50MPa. We also show that the isotropic-nematicphase transition of pentyl-cyanobiphenyl (5CB) liquid crystal provides asimple, convenient and accurate method for calibrating pressure in the range0.1-200MPa.

A multi-purpose modular system for high-resolution microscopy at high hydrostatic pressure