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We describe a gradiometer based on a high-transition temperature Superconducting Quantum Interference Device (SQUID) for improving the sensitivity of a SQUID-based biosensor. The first-derivative gradiometer, fabricated from a single layer of YBa{sub 2}Cu{sub 3}O{sub 7-x}, has a baseline of 480 {micro}m and a balance against uniform fields of 1 part in 150. Used in our SQUID ''microscope,'' it reduces parasitic magnetic fields generated by the measurement process to the level of the SQUID noise. The gradiometer-based microscope is two orders of magnitude more sensitive to super paramagnetic nanoparticles bound to biological targets than our earlier magnetometer-based microscope

Magnetic gradiometer based on a high-transition temperature superconducting quantum interference device for improved sensitivity of a biosensor