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Producing of a collagen preparation for determining platelet functions requires the testing of the activity of each single batch to make possible a standardization. For this purpose methods using PRP (platelet rich plasma) to measure the platelet aggregation are not very appropriate. Since four years a collagen centrifugation test proves to be suitable for determining the activity of various batches of Collagenreagent “Horm”. In this test the platelets of citrated whole blood are mixed with graduated doses of collagen directly by the withdrawal of blood. The turbidimetrical evaluation of the PRP obtained from the collagen treated whole blood samples shows 15% (SD ± 3. 5 %) of the O.D. (optical density) of the PFP (platelet free plasma) after a dose of 1 meg and 80% (SD ± 2 %) after a dose of 2 meg collagen per ml whole blood. The aggregation of platelets in citrated whole blood simultaneously with graduated doses of collagen allows to standardize different batches of platelet specific collagen preparations.

Testing of the Aggregating Activity from Various Batches of a Platelet Specific Collagen Preparation