English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

In normal prothrombin, 10-glutamyl residues present in the amino portion of the molecule are carboxylated to form γ-carboxyglutamic acids (gla). Dicoumarol, an antagonist of vitamin K, induces the production of (partially) acarboxylated atypical prothrombins. In addition to our atypical varieties, viz. 7−,5− and 2-gla prothrombins, we have isolated two more atypical molecules, one containing 1.0±0.1 gla (1-gla prothrombin) and the other approximately 0.25 gla (0-gla prothrombin). These two variants adsorbed onto alumina Cγ-gel (Bio-Rad), similar to 2-gla protein, but were derived from 40 to 50% (NH4)2SO4 saturation. The purified materials, obtained after isoelectric precipitation followed by preparatory polyacrylamide-gel electrophoresis and heparin agarose chromatography, showed a single component by analytical disc-gel electrophoresis both in the presence or absence of sodium dodecyl sulfate (SDS) and contained antigenic activity comparable to that of normal prothrombins.  The pI’s of the two variants by column electrofocusing were each 4.835±0.015. Similarly, the two proteins did not reveal any difference in electrophoretic mobility; however, their prothrombin fragments 1 (F1, residues 1-156) did—0-gla F1 moved slower than 1-gla F1. Employing anti (normal) prothrombin sera with Ca2+ , the two, 0− and 1-gla, F1’s produced vaguely visible immunoprecipitates which were definitely lighter than all the other F1’s including 2-gla. These results confirm that not only are multiple forms of atypical prothrombin induced by dicoumarol but also that gla does affect the immunochemical properties of the gla-containing fragment.

Purification Of 1- And 0-Gla Prothrombins And Their Differentiation With Normal And Other Dicoumarol-Induced Prothrombins