English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The fact that high shear activates and damages platelets has been suspected to be a major cause of thromboembolism in artificial internal organs (AIO) or in arterial stenosis. In AIO wall shear stresses well above 50 Nm-2 have been computed to which blood cells are exposed for times in the order of milliseconds (ms). Unfortunately, the studies on this subject employing defined flow conditions operate with exposure times higher than 10 seconds. The pupose of the present study was to elucidate the effects of high shear exposure for ms upon platelet function (ADP induced platelet aggregation (PA), platelet procoagulant activity {PF- 3)) under heparin anticoagulation, which is also used in AIO.  To apply shear rates ranging from 50 - 220 Nm-2 to heparinized PRP for defined exposure times between 7 - 700 ms a flow through Couette-viscometer was employed. Platelet factor 3 (PF-3) availability was estimated by using a modified Stypven time technique. Lactic dehydrogenase (LDH) liberation was taken as a measure for platelet destruction. All steps of the experimental procedure were carried out at 37°C.  From our data we conclude that, in the presence of physiological calcium levels (heparin anticoagulation) shear stresses and exposure times that certainly occur in AIO are able to activate platelets and procoagulant potential of blood.

Effects Of Short Time High Shear Exposure Upon Platelet Function And The Coagulation System Under Heparin Anticoagulation