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In our preliminary studies dealing with bovine plasma lipoproteins, we suggested that the spectrum of particles with d> 1.026 g/ml could be divided into LDL (d 1.026-1.060 g/ml), light HDL (HDL L d 1.060-1.091 g/ml) and heavy HDL (HDL d 1.091-1.180 g/ml) (Bauchart et al, 1989). However, subfractions corresponding to the d 1.040-1.090 g/ml range contain 2 types of lipoproteins with characteristics of LDL and HDL particles, respectively. Indeed, their separation by affinity chromatography on a heparin!epharose column showed the presence of 2 forms of HDL containing apoprotein (apo) A-I and one form of LDL containing apo B with only slight traces apo A-I (Laplaud et al, 1989). Therefore, we directly estimated the respective density distributions of LDL and HDL particles in bovine plasma and lymph by radial immunodiffusion (RID) assays of apo A-I and apo B. A 3 wk old preruminant calf (46 kg, 600 g/d body weight gain) was fed a milk substitute

reproduction nutrition development/annales de biologie animale, biochimie, biophysique/reproduction nutrition développement

Determination of the respective density distributions of low-and high-density lipoprotein particles in bovine plasma and lymph by immunoassay of apoproteins A-I and B