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Pieces of 6and 8-day male and female left embryonic chicken gonads were cultured in vitro for 17 or 21 days in ovo with and without androstenedione (0), dihydrotestosterone (DHT) and estradiol 17 p(E! p). Control cultures of female gonads differentiate into typical ovaries. In the cortex, the prefollicular cells elaborate « lining bodies>> and the germ cells develop Balbiani bodies, intercellular bridges and meiotic prophase figures. The ovarian cortex of treated female gonads is similar to that of controls. Control cultures of male gonads differentiate into typical testes. No cortical tissue is observed. In most cases, treatment with E. p, 0, or DHT induces a partial or complete sexreversal in the male gonads. In the cortex feminized by E, p or 0, the fine structure of prefollicular and germ cel Is is similar to that in females. Although DHT produces an extensive

reproduction nutrition development/annales de biologie animale, biochimie, biophysique/reproduction nutrition développement

Fine structure of prefollicular and developing germ cells in the male and female left embryonic chick gonads in vitro with and without androgenic steroids