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The effect of 25 OHD 3, 1.25 (OH) 2D3, 24, 25 (OH) 2D3and 25, 26 (OH) 2D3was studied on the bone of vitamin D-deficient rats. The products were administered by intraperitoneal route during 5 days at a dose of 325 pmol/day. The animals were killed 24 hours after the last injection. Serum calcium was significantly increased by all metabolites, but the increment was not similar in all cases ; serum phosphorus was only increased by 25 OHD 3 and 1.25 (OH) D3. Bone calcium content increased after all treatments, but the bone phosphorus content augmented only after 25, 26 (OH) 2D, treatment. Histological studies showed that 25 OHD 3 had an osteoblastic action and 1.25 (OH) 2D3an osteoclastic action. 24, 25 (OH) 2D3calcified the osteoid and increased the number of osteoclasts, but not their activity. 25, 26 (OH) 2D, simulated calcification without any increment of osteoclastic activity. The bone of 25, 26 (OH) D3-treated rats was similar to the bone of normal animals.

Les métabolites de la vitamine D : leur action sur l'os des rats carencés en vitamine D