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The induction of estrus ovulation and fertility in prepuberal gilts was utilized to study factors influencing fertility and early embryonic development. 5 mg gonadotropin-releasing hormone (GnRH) was not effective in causing either estrus or ovulation. An injection of either 5 or 10 mg GnRH plus 300 IU pregnant mares serum gonadotropin (PMSG) resulted in ovulation in less than 50% of the gilts. Treatment with 200 300 400 or 500 IU PMSG plus either 200 or 300 IU human chrionic gonadotropin (HCG) resulted in ovulation in 95% of 80 animals treated. Laparotomy indicated that most ovulations took place between 110-120 hours postinjection regardless of the dose. The gilts were sacr ificed 7 days postinjection and 3 days postinsemination. Significantly fewer (p less than .01) ovulations were seen in gilts injected with 200 IU than with 300 or 400 IU PMSG. When the dose of HCG was increased fro m 200 to 300 IU ovulation rate (p less than .05) and ovarian weight (p less than .01) were increased while uterine weight (p less than .01) was reduced. Implants or single injections of 40 mg ethinyl estradiol resulted in estrus and ovulation in a majority of the females treated.

INDUCTION OF ESTRUS, OVULATION AND FERTILITY IN PREPUBERAL GILTS