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The mosquito species  Aedes  ( Ochlerotatus )  coluzzii  Rioux, Guilvard &amp; Pasteur, 1998 was distinguished from its sibling species  Aedes detritus  (Haliday, 1833) using an isoenzymatic method that required the destruction of the entire specimen, therefore no holotype was designated by the species authors. We aimed to designate a neotype for  Ae. coluzzii  from specimens collected from the type-locality and individually reared up to adult stage. Genomic DNA was extracted from pupal exuvia and ITS2 was sequenced, enabling verification of the identity of each specimen as  Ae. coluzzii  or  Ae. detritus . Among the series of  Ae. coluzzii , a male was designated as neotype and deposited in a collection. To our knowledge, this is the first time the type of a mosquito species is deposited thanks to its molecular identification from its pupal exuvia. The set of identified specimens allowed additional phylogenetic and morphologic studies.

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Using a pupal exuvia to designate the undamaged neotype of a species belonging to a complex of sibling species – the case of <i>Aedes coluzzii</i> (Diptera, Culicidae)

Using a pupal exuvia to designate the undamaged neotype of a species belonging to a complex of sibling species – the case of Aedes coluzzii (Diptera, Culicidae)