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This study investigated the effects of  Kudoa septempunctata  genotype ST3 spores on ddY suckling mice. Purified  Kudoa septempunctata  spores were administered into the stomachs of the mice at 5 × 10 6  or 5 × 10 7  spores/mouse, with inactivated  Kudoa  (5 × 10 6  spores/mouse) or vehicle as controls. No abnormal clinical symptoms were observed and there were no variations in fluid accumulation ratio and cytokine gene expression in all groups. In addition, intact  Kudoa  spores and the 18S rDNA gene were only detected (by microscopy and quantitative PCR, respectively) in the groups administered such spores. This study thus confirms that spores from the ST3 strain of  Kudoa septempunctata  were excreted in the faeces without infecting the gastrointestinal tract in ddY suckling mice.

Effects ofKudoa septempunctatagenotype ST3 isolate from Korea on ddY suckling mice