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We conducted a survey in broiler farms from Romania to establish prevalence and distribution of  Eimeria  species using single PCR assay. We found  Eimeria  spp. in 21 (91%) out of 23 flocks, and in 11 (92%) out of 12 farms. Four species of  Eimeria  were identified:  E. acervulina  (21/23; 91%),  E. tenella  (14/23; 61%),  E. maxima  (5/23; 22%) and  E. praecox  (3/23; 13%). Infection with a single species ( E. acervulina ) was detected in 6 (26%) infected flocks originated from large farms. Mixed infections were found in 15 (65%) flocks and the most prevalent combination was  E. acervulina  +  E. tenella  (8/23; 35%). Four flocks (17%) harboured mixed infection with  E. acervulina + E. tenella  +  E. maxima .  E. acervulina  was significantly more prevalent in flocks that received ionophores as anticoccidial feed additives.

Prevalence and distribution ofEimeriaspecies in broiler chicken farms of different capacities