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To perform IgG avidity immunoblotting assay for detection of acute toxoplasmosis, 100 serum samples were collected from Tehran, Iran. The presence of  Toxoplasma -specific IgG and IgM antibodies were checked by commercial Trinity kit. Samples were categorized in acute and chronic phases of  Toxoplasma gondii  infection according to IgG avidity ELISA. IgG avidity immunoblotting was performed, and antigenic bands with molecular weights of 22, 25, 28, 30, 32, 42, 44, 49, 55, 60, 66, 69, 88, 106, 130 and 157 kDa were recognized as low avidity markers. The most prevalent antigen for low avidity was p22. It is concluded that IgG avidity immunoblotting could distinguish acute and chronic phases of  T. gondii  infection.

Diagnosis of antigenic markers of acute toxoplasmosis by IgG avidity immunoblotting