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The application of the Simplex optimization procedure allows in functions of the form I(E) = A*E-r, A and r values to be calculated. The use of element-containing Bio-standards with a known externally determined concentration aids in getting reproducible results. Due to the reproducible spectra, fitting-procedures can be compared. The application of a three-dimensional Simplex optimization allows three parameters mutually to be compared. In this way the minimum length of the fitting zone (0393) can be determined in order to find a constant value of Rx.

microscopy, microanalysis, microstructures

Comparison of the simplex method with several other methods for background-fitting for electron energy-loss spectral quantification of biological materials