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2 This study focused on the removal of sulphide from liquid solution using biofilm on packed bed of salak fruit seeds. Bio-filter operation of 444 hours consists of 6 phases of operation. Each phase lasted for approximately 72 hours to 82 hours and run at various inlet concentration and flow rate. The highest removal efficiency is 92.01%, at the end of phase 7 at the inlet concentration of 60 ppm and the flow rate of 30 mL min-1. Mathematic model of sulphide removal was proposed to describe the operation of bio-filter. The model proposed can be applied to describe the removal of sulphide liquid using bio-filter in packed bed. The simulation results the value of the parameters in process. The value of the rate maximum specific growth is 4.15E-8 s -1 , Saturation constant is 9.1E-8 g cm -3 , mass transfer coefficient of liquid is 0.5 cm s -1 , Henry’s constant is 0.007, and mass of microorganisms growth to mass of sulphide consumed is 30. The value of the rate maximum specific growth in early process is 0.4 s -1 .

Sulphide removal from liquid using biofilm on packed bed of salak fruit seeds