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A critical comparison of four techniques used to estimate the abundance and biomass of bacteria associated with leaves decaying in a small river in the North-East of Morocco is given. The relative efficiency of the four methods was as follows: homogenisation > direct count > stomacher > sonication. However, each one of these techniques presents its advantages and disadvantages. No technique is perfect by itself.

A critical comparison of techniques for estimating bacterial abundance on leaf litter decaying in Oued Zegzel (Morocco)