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Summary - A microparticle-enhanced nephelometric immunoassay of bovine immunoglobulins G is reported. lt is based on the nephelometric quantification of the competitive immunoagglutination of covalently coated microparticles. This new immunoassay is easy to perform (1-step, no sample pretreatment, no washing or phase separation), fast (1 h at most), sensitive (8 Ilgl1 of immunoglobulins G significantly detected in the reaction mixture), accu rate (linear recovery of immunoglobulins G in overloaded milks) and reproducible (CVs from 2.3-10% in within- and between-run precision studies). The potential of this microparticle-based immunoassay ls discussed with regard to the other quantitative immunoassays (radial immunodiffusion and convention al immunonephelometry in parneular) used in the determination of milk immunoglobulins G.

Microparticle-enhanced nephelometric immunoassay for immunoglobulins G in cow's milk