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road samples were collected from 4 dairy plants to evaluate the analytical performance of different methods of measurement of A TP content used to assess the bacteriological quality of milk. Ali sampIes were analysed in duplicate by Spiral standard reference method. One hundred and twenty sampIes were analysed in duplicate by rapid methods to determine ATP level by bioluminescence: the ATP platform test (ATP-p) and L1ATP test (L1ATP). Ninety-four other samples were tested by a new method including a filtration step: the A TP-F test (A TP-F). The geometric relative standard deviation of repeatability (in % CFUlml) was 14% for the ATP-p test, 51% for the L1ATP test and 43% for the ATP-F test. The residual standard deviations from the regression of the reference method on the ATP level were 0.637 and 0.623 log CFUlml respectively for the ATP-p and the MTP (not significantly different at the 5% level). The ATP-F test residual standard deviation was much lower (Sy,x = 0.301 log CFUlml), and the slope of the regression line was significantlyabove 1.000 at the 5% level.

Comparaison de trois méthodes de dosage de l'ATP par bioluminescence pour l'estimation de la qualité bactériologique du lait cru