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Abnormal heat resistance of bacterial spores heated by direct injection into steam Spores of Clostridium tyrobutyricum, Bacillus cereus and B. stearothermophilus were heated in a pilot-plant D.H.T. sterilizer, of the milk-into-steam type. Temperatures were chosen between 102 and 115°C, and the mean holding time was about 10 sec. Working fluids were buffer, whole milk or skim milk. Distribution function for residence times was determined by means of methylene blue as a tracer. Theoretical sporicidal effectiveness was calculated for each strain and each temperature, using heat resistance values previously determined by heating spores in T.D.T. tubes. With Cl. tyrobutyricum and B. cereus the experimental sporicidal effectiveness was always higher, fram 1.2 ta 7 times, than the computed effectiveness. Furthermore, with Cl. tyrobutyricum spores, the T.D.T. curve is modified : z increases (13° C instead of 9.8° C in T.D.T. tubes.) B. stearothermophilus spores heated in the same D.H.T. sterilizer were partially inactivated at a temperature at which heating in ampoules results in an activation.

Thermorésistance anormale de spores bactériennes chauffées par injection directe dans la vapeur