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Increasing the river habitat safeguard level is one of the main actions proposed by theEuropean Water Framework Directive in the field of biomonitoring. To do so, watercourseswithin the same hydroecoregions (that is, homogeneous areas based on climate, geology andtopography) ought to be compared. In addition, the source distance was thought to play animportant role in comparing rivers and then planning monitoring activities. The purpose ofthis study was to evaluate if both hydroecoregion and source distance affect the response(in terms of taxa assemblage) of one of the most used group in the river monitoringactivities: the benthic macroinvertebrate. Here we proposed the comparative influence ofhydroecoregion and source distance on the invertebrate assemblage in Mediterranean riversof central Italy. Our statistical outputs highlighted how macroinvertebrate differed forboth hydroecoregions and source distance ranks. In particular, no differences were foundamong sites of different (1) source distance ranks and (2) hydroecoregion (that is, whenthis two descriptors were analyzed separately), while the highest difference in themacroinvertebrate assemblage was observed between the same source distance ranks ofdifferent hydroecoregions. Our results showed how the use of both hydroecoregion andsource distance should be considered for planning monitoring activities to properly managerivers and water resources

Assessing the influence of source distance and hydroecoregion on the invertebrate assemblage similarity in central Italy streams