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The diet and food niche overlap of five fish species in the Úpoř brook was evaluated. Thesupply of food represented by benthos was studied, and the proportion of total foodparticles in the intestine of brown trout Salmo trutta L., chubSqualius cephalus (L.), dace Leuciscus leuciscus (L.),bullhead Cottus gobio (L.) and stone loach Barbatula barbatula(L.) was measured. Evaluations were performed using a new index(Mtotp). All fish species except stoneloach exhibited a balanced consumption of the food supply. The food components weresystematically grouped, but no differences in the ingestion of these groups were found.However, a new approach in the evaluation of food competition based on observation ofspecies-specific preferences/avoidance in ingestion of food particles was applied. Theparticles were divided into four ecological categories according to their availability forfish. Brown trout concentrated on easily accessible sources of benthos or that hidingbelow stones, and also distinctly preyed on terrestrial insects. Chub displayed a similarfeeding habit, though concentrating more on drift and allochthonous sources. The foodspectrum of dace was mostly composed of easily accessible benthic organisms, consumingfewer hidden benthos and terrestrial insects. The food of bullhead and stone loach wassimilar, both preferring benthos. Bullhead consumed hidden species while stone loachconsumed more accessible species rather than hidden ones

The food niche overlap of five fish species in the Úpoř brook (Central Bohemia)