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An analytical technique to investigate the chemical state of trace metals in a small amount of sample has been proposed. While the principle for distinguishing the chemical state is based on well known X-ray spectroscopic phenomena, i.e., chemical shifts of the absorption edges, a total reflection mirror has been employed as a support to enhance the signal to background ratio through the reduction of scattering X-ray background. The present paper describes the details of the analytical procedure and shows the successful results of the near edge absorption spectrum of trace iron (~ mM) in a wet liquid drop (~ µl) measured in 3 ~ 5 min. The feasibility of the application to biomedical science is also mentioned

Trace Chemical Characterization of Liquid Drop by Fluorescence Detection of Absorption Edge Shifts using Total Reflection Support