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Experiments were performed with needles from Norway spruce trees (Picea abies L.) growing in the field. Extracts of spruce needles were prepared as described elsewhere (Polle et al., 1989). After dialysis, the activity of superoxide dismutase was determined according to the method of Misra and Fridovich (1972). This assay is based on the autooxidation of epinephrine to adrenochrome at pH 10.2. 0! serves as the chain-propagating species in this reaction. SOD competes for 02, thus inhibiting adrenochrome formation. By definition, 1 unit of SOD is the amount of extract that inhibits the maximal rate of adrenochrome formation by 50%.

Factors modulating superoxide dismutase activity in needles of spruce trees (Picea abies L.)