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The work concerns the feasibility of 3D optical diagnostic of porous media with subdifraction spatial resolution via epi-luminescence microscopy of single semiconductor colloid nanocrystals (quantum dots, QD) CdSe/ZnS used as emitting labels/nanoprobes. The nanoprecise reconstruction of axial coordinate is provided by double helix technique of point spread function transformation (DH-PSF). The results of QD localization in polycarbonate track membrane (TM) is presented

3D imaging of semiconductor colloid nanocrystals: on the way to nanodiagnostics of track membranes