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About 30% of all proteins contain metals either as cofactor or covalently bound as part of the protein. Some of these metalloproteins such as transferrin (TRF) and haemoglobin (HGB) are regularly measured in clinical laboratories. However, for most of them, no primary reference measurement procedures exist, although directives such as the EC-directive covering in vitro diagnostic medical devices ((IVD-) directive 98/79/EC) and standards such as EN ISO 17511:2003 demand the traceability of results received for analytes in samples of human origin. The EMRP project HLT05 aims at establishing a traceability chain for metalloproteins as is already in place for some inorganic and small organic molecules in biological samples

Metrological Approach for the Quantification of Metalloproteins (EMRP Project HLT05)