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The mtDNA sequences revealed that several of the fish studied were  Hampala macrolepidota  and  Barbonymus gonionotus . The objective of this research was to learn the pattern of COI gene in mtDNA and establish a phylogenetic tree. Basic Local Alignment Search Tool-nucleotide (BLASTn) confirmed that  Barbonymus gonionotus  froma the Ranau Lake, South Sumatera has 100% matching ranges to the species from Memberamo River (Indonesia), India, Bangladesh, Thailand (Mae Khlong), Indo-Myanmar, and Malaysia_1. The lowest closeness (98.76%) is related to species from Thailand (Lower Ing). The Blast investigation appears us that the level of familiarity was very high, it is coming to 98-100% in  Barbonymus gonionotus .  Hampala macrolepidota  had 100% matching ranges to the species from Indonesia (SouthaSumatera_1) and Vietnam. They had 99.05%-99.84% closeness from Malaysia_1,2&amp;3, Indonesia (South Sumatera_2&amp;3, Java and Bali_1,2&amp;3).

Fish Identification based on Partial Fragments of The Mitochondrial COI Subunit I Gene