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Lecithin in mixed micelles attenuates cytotoxicity by interacting with proteins or other cell inclusions. The reaction was then captured via magnetic beads binding approach. This research proposal was designed to discover the impacts of lecithin on apoptosis relevant factors (including caspase 3/8/9 and PARP) by Western Blot and specific protein binding mechanism of lecithin detoxification pathway, using caco-2 cell model. Preliminary investigation of the properties of the proteins was conducted by MS (Mass Spectrometry) couplet to AC (Affinity Chromatography) assay. Further research of particular proteins was to identify the size and peptide sequence. This study also provided solid evidence for the previous research result, which was lecithin reduce cytotoxicity induced by SDC contained mixed micelles.

Responses to Higher Concentration Lecithin of Apoptosis Relevant Factors and Detection of Specific Lecithin Binding Proteins by AC-MS assay