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PAL ,  UFGT  as structural genes and  MYB10  as a regulatory gene play an important role in the accumulation of anthocyanins in plants. In this experiment, ‘Cuihongli’ and ‘Qiangcuili’ were used as materials to clone  PAL ,  UFGT  and  MYB10  related to anthocyanin synthesis by homologous sequence cloning. The results showed that the full length of  PAL  was 2160 bp, encoding 719 amino acids; the full length of  UFGT  was 1428 bp, encoding 475 amino acids, and the differences of PAL and UFGT between the two cultivars were one amino acid and three amino acids, respectively. The sequence length of ‘Cuihongli’  MYB10  gene is 732 bp, which encodes 243 amino acids and belongs to the superfamily of SANT family. Homology analysis and phylogenetic tree analysis showed that the proteins encoded by  PAL ,  UFGT  and  MYB10  genes were short and closely related to  Prunus persica ,  Prunus avium ,  Prunus armeniaca  and  Prunus cerasifera  in Rosaceae.

Cloning of MYB10, PAL and UFGT genes from ‘Cuihongli’ and ‘Qiangcuili’