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Along with the increasing quantity of agriculture in Indonesia, should be followed by improving the quality of agriculture. Generally one of the indicators or parameters commonly used to indicate the quality of agriculture is the number of pests that damage plants in certain areas. The control of disturbing organisms on the plant can be minimized by the bio-insecticide production of Carica papaya which is tapped and extracted with ultrasonic-assisted using a mixed NADES solvent from Cholin chloride (ChCl) and Oxalic acid (Ox) with varying sonification time for 10, 20, 30 minutes and solvent ratio of 1: 2 and 2: 1. Bio-insecticide was produced by conducting qualitative enzyme activity of with lowry method and quantitatively using UV / Vis spectrophotometer aid with 750 nm wavelength and efficacy test. The best extraction result was obtained at NADES solvent with 1: 2 ratio with extraction time for 30 min with extraction time for 30 minutes yielding protein concentration equal to 7,23 ppm and tyrosine concentration equal to 7,9 ppm. Bio-insecticide samples effectively kill grayak caterpillars with 100% mortality within 5-7 days.

The Effect of NADES Ratio Solvent and Sonication Time in Extraction of Papaya Sap for Bio-insecticide Production