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The article presents the results of studying the radio protective properties of alfalfa extract (dietary supplement "Erakond"). A decrease in the iron content in macrophages of the red pulp of the spleen was found experimentally using models of experimental radiation pathology induced by ɣ-irradiation of white rats in a single dose of 4 Gy at a dose rate of 1.7 Gy/min. The mechanisms of pathological processes in acute radiation pathology, leading to a decrease in the content of endogenous iron at the systemic, organ, cellular and subcellular levels, have been disclosed. Violation of the processes of enteral absorption of minerals as a result of pronounced destructive-inflammatory processes in the intestinal wall, damage to the hematopoiesis-inducing environment in the red bone marrow, inhibition of cytokine synthesis (IL-1, IL-3, IL-6, IL-7) and granulocytic macrophage colony-stimulating factor (GM-CSF) reduce the level of deposited iron in acute radiation pathology. In rats treated with 40 % "Erakond" aqueous extract before and after irradiation, there is a relatively high level of deposited iron in the spleen tissues in comparison with animals of the comparison group, which were on a general food ration.

Identification of iron by methods of cytochemical analysis in the spleen tissues during experimental radiation pathology