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The results of the analysis of allelic polymorphism of the  Rpf1  red stele root rot resistance gene in strawberry hybrid forms using diagnostic DNA markers were shown. The recessive homozygous genotype ( rpf1rpf1 ) was identified in strawberry seedlings 932-29 ( F. virginiana  subsp.  platypetala  (Rydb.) Staudt × Feyerverk), 933-4 ( F. virginiana  subsp.  platypetala  (Rydb.) Staudt × Rubinovyy kulon), 62-7, 62-23 (Bylinnaya × Feyerverk), 65-2, 65-15 (Olimpiyskaya nadezhda × Bylinnaya) and 35-16 (922-67 × Maryshka). The heterozygous state of the  Rpf1  gene was identified in strawberry forms 69-29 (Feyerverk × Bylinnaya), 62-41 (Bylinnaya × Feyerverk), 72-24, 72-71 (Privlekatelnaya × Bylinnaya), 65-17, 65-24 (Olimpiyskaya nadezhda × Bylinnaya), which allows them to be used in breeding for resistance to  P. fragariae  var.  fragariae  as valuable initial forms.

Marker controlled screening of resistant to red stele root rot (Rpf1 gene) strawberry selected forms