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Article, pickle, and wet blue leather defects used for this study were fromthe Balai Besar Karet college, Kulit dan Plastik, D.I Yogyakarta (BBKKP YK), Indonesia. Meanwhile, the microorganisms in leather defects were grown  in vitro  at A-minimal mineral (MM) and B-lowest (1/200 v/v) nutrient media. A nitrogen source of 2% Sigma-Aldrich bovine gelatine was added to each medium. Furthermore, 1cm 2  of each leather defect was sliced and immersed into the in vitro media for 7 days in an open-air rotary incubation with ambient temperature at 28° C to 30° C. The first or conventional method was the rubbing of ose cotton into the solid media, while the second isolation method was the centrifugationof liquid growth medium at 15.1G for 20 minutes. Moreover, the four microbial isolates were fromglossy yellow colonies A and B as we l  as white colonies. These colonies were incubated at 38° C and the four microbes produce proteases after growing for at least 7days in liquid media and 24 hours less in solid media. The protease test produced gases on the pickle leather defects using a test tubeglass of 0.8cmdiameter and 15cmlong. Therefore, the chemical tanning process on leather defects creates a unique ecosystem of microorganisms as collagen proteins change and become thekeyto their growth.

Future Benefits of Microorganism on Leather Defects in The Industrial Production of Protease