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Vanda tricolor  Lindey var.  Suavis  is an endemic orchid from Mt.Merapi, Yogyakarta Special Region, Indonesia. This orchid has beautiful flowers with unique patterns of white and purple spots, fragrant and can live in the slopes of Mt.Merapi which is a very active volcano. UGM in collaboration with the Netherlands carried out ex situ conservation of the  V. tricolor  Merapi through the self-pollination of orchids by the Queen of the Netherlands, Queen Maxima on March 11, 2020 at the UGM campus, Yogyakarta. In honor, the new generation of crosses is named  Vanda tricolor  var  Suavis  ‘Queen Maxima’. This study aims to produce mass quantities of the orchid and characterize the  HSP70  gene in it. Methods: Standard  in vitro  culture for Vanda on MS, NP and VW medium, isolation of plant gDNA, PCR with V. tricolor HSP70primers and sequencing of amplicon DNA. The results showed that &gt; 90 % of  V. tricolor  ‘Queen Maxima’ seeds germinated well in all media and the best on VW medium.  V. tricolor  has the HSP70 protein with PTZ00009 super family amino acid motif that 87 % similar to the HSP70 protein from the  Phalaenopsis equestris  orchid, which is probably the reason  V. tricolor  become superior to high temperature environments.

In vitroculture and characterization of theHSP70gene onVanda tricolorLindley var.Suavis‘Queen Maxima’