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DNA markers are widely used in grapevine breeding to create forms with combined resistance genes. Downy mildew is one of the most common fungal diseases of the vine in the world. Growing grapevines with increased resistance allows to reduce the number of chemical treatments. The decrease in the use of pesticides is especially significant for viticulture of table varieties, since berries are directly consumed by humans for food. Currently, more than 20 resistance genes have been identified by molecular methods, and DNA markers for many genes have been developed. The genes  Rpv3  (inherited from North American grape species) and  Rpv12  (derived from  V. amurensis ) are among the most effective and have an additive effect. The study of 14 table grape varieties for the presence of the  Rpv3  gene and 8 varieties for the presence of the  Rpv12  gene was performed by using DNA-marker analysis. The analysis included varieties that could inherit these genes from the parent forms, according to their ancestry. The study was conducted using an automatic genetic analyzer ABI Prism 3130 and special software GeneMapper and PeakScanner, DNA-markers were taken from literature sources. According to the results of DNA-marker analysis, 9 varieties were identified, including 2 seedless varieties, with the  Rpv3  299-279  allele in the genotypes, which determines resistance to downy mildew, and 3 table varieties with the  Rpv12  gene in the genotypes. One table grape genotype was identified with  Rpv3  and  Rpv12 .

DNA-marker identification of Rpv3 and Rpv12 resistance loci in genotypes of table and seedless grape varieties