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International audiencePaenibacillus larvae larvae is the causative agent of American foulbrood (AFB), a serious honeybee disease. The ability to detect and identify this organism promptly is important to allow effective sanitary measures in case of AFB. P. larvae larvae is a fastidious and slow-growing bacterium and primary isolation and presumptive identification can be difficult and time-consuming. In this study the use of PCR is described for a rapid and reliable diagnosis. The developed PCR assay is specific for P. larvae as no amplicons were produced from 13 related or hive-related species. Only with P. larvae larvae and P. larvae pulvifaciens DNA as template, an amplicon was formed. A positive reaction was also observed when DNA was extracted directly from remains of an AFB diseased larva. Thus this PCR assay provides a reliable diagnosis for AFB in only 4 hours

apidologie

Development of a fast and reliable diagnostic method for American foulbrood disease (<i>Paenibacillus larvae</i> subsp. <i>larvae</i>) using a 16S rRNA gene based PCR

Development of a fast and reliable diagnostic method for American foulbrood disease (Paenibacillus larvae subsp. larvae) using a 16S rRNA gene based PCR